Abstract: Objective To explore the model of cyclophosphamide-induced reproductive damage in male mice from two aspects of animal species and cyclophosphamide dosage. Method Healthy six-week-old male ICR mice and BALB / c mice were randomly divided into 8 groups: ICR-control, ICR-60, ICR-100, ICR-200, BALB-control, BALB-60, BALB-100 and BALB-200. ICR-control and BALB-control groups were intraperitoneally injected with normal saline once a week for 5 weeks. ICR-60 group and BALB-60 group were intraperitoneally injected cyclophosphamide 60 mg / kg every day in the first 5 days. ICR-100, BALB-100 groups and ICR-200, BALB-200 groups were intraperitoneally injected with cyclophosphamide 100 mg / kg and 200 mg / kg once a week for 5 weeks, respectively. The experiment lasted for 35 days. The mice were weighed weekly and the death of the mice was recorded. On the 36th day, the mice were sacrificed, the testes and epididymis were separated, weighed, and the testis and epididymis indexes were calculated. One side of testis was fixed, embedded and sectioned, and the testis structure was observed by HE staining. The other testis was frozen, and the activity of superoxide dismutase was measured. Result The mortality of BALB-60 and BALB-200 groups was 100%, the mortality of ICR100, ICR-200 and BALB-100 groups was high, and the mortality of ICR-60 group was 0. The testis index and epididymis index of ICR experimental groups decreased to different degrees, but only the testis index of BALB-100 group decreased. HE staining showed that spermatogenic tubules were damaged in all model groups, and the degree of damage was correlated with cyclophosphamide concentration. SOD activity of testis in ICR-60 group decreased, but SOD activity of testis in other groups did not change significantly. Conclusion ICR-60 is a more suitable model of cyclophosphamide-induced mice reproductive damage.

Key words: cyclophosphamide, reproductive damage, mice, testis

摘要： 目的 从实验动物品种和环磷酰胺给药剂量两方面,探索环磷酰胺致雄性小鼠生殖损伤的模型。 方法 健康 6 周龄雄性 ICR 小鼠和 BALB / c 小鼠,随机分为 8 组:ICR-control、ICR-60、ICR-100、ICR-200、BALB-control、BALB60、BALB-100、BALB-200 组。 ICR-control、BALB-control 组腹腔注射氯化 钠 溶 液,每 周 1 次,共 5 周。 ICR-60 组 和BALB-60 组实验于前 5 d 每天腹腔注射环磷酰胺 60 mg / kg;ICR-100、BALB-100 组和 ICR-200、BALB-200 组分别给予 100 mg / kg、200 mg / kg 环磷酰胺腹腔注射,每周一次,共 5 周。 实验周期 35 d,小鼠每周称重,记录小鼠死亡情况。 第 36 天处死小鼠,分离睾丸和附睾组织,称重并计算小鼠睾丸和附睾指数,一侧睾丸固定、石蜡包埋、切片,HE染色,观察睾丸组织结构,另一侧睾丸冷冻,测定超氧化物歧化酶活性。 结果 BALB- 60、BALB-200 组小鼠死亡率为 100%,ICR-100、ICR-200、BALB-100 组死亡率较高,ICR-60 组死亡率为 0。 ICR 实验动物模型组睾丸指数和附睾指数均有不同程度的降低,BALB-100 组仅有睾丸指数下降。 HE 染色显示,各模型组均造成生精小管损伤,损伤程度与环磷酰胺浓度相关。 ICR-60 组小鼠睾丸 SOD 活性降低,其他组睾丸 SOD 活性无明显改变。 结论 ICR-60 为较为合适的环磷酰胺小鼠生殖损伤模型。

关键词: 环磷酰胺, 生殖损伤, 小鼠, 睾丸